Vibrant by UWC testing

twilliard · Dec 29, 2016

Love the sub!

jason2459 · Dec 29, 2016

twilliard said:
Love the sub!

That was a Christmas present I just got from my son. So, it will be featured up front for a while. If you notice in some pictures of the goniopora there's a dragon head by it. Bottom part of the jaw has worked it's way under the sand. That was a Christmas present from my son a few years ago. lol

Brew12 · Dec 29, 2016

jason2459 said:
That was a Christmas present I just got from my son. So, it will be featured up front for a while. If you notice in some pictures of the goniopora there's a dragon head by it. Bottom part of the jaw has worked it's way under the sand. That was a Christmas present from my son a few years ago. lol

Glad the sub I was on never looked like that!

jason2459 · Dec 30, 2016

Hmm, I just noticed the algae area that I haven't touched above the pump looks cleaner. From at least this view. I didn't really look at it last night from the inside.

There's actually spots that can be seen through. Will check and post pictures of that spot tonight.

blazedmasta · Dec 30, 2016

When dosing once a week is anyone having issues with keeping micro algae in a refugium? Like cheato.

Brew12 · Dec 30, 2016

blazedmasta said:
When dosing once a week is anyone having issues with keeping micro algae in a refugium? Like cheato.

I lost my cheato dosing 2x per week. Sounds like it can hold on for awhile but not long term at more than 1 dose every 2 weeks.

btw... Cheato is a macro algae, not micro algae.

jason2459 · Dec 30, 2016

that algae is not nearly as dense on top of the powerhead now

drtrash · Dec 30, 2016

Just started 1x week have a baseball size in fuge will see in a week hope to have cheato and weekly treatment or at least every other week soon

jason2459 · Dec 30, 2016

Yeah, I don't know myself what it will do with macro algaes as I didn't have any. Seems ulva is pretty resilient against it but chaeto is not according to some other posts.

I'm due to clean my ATS that grows micro algae well and wasn't impacted two weeks ago. Will see now and again in two weeks.

NanaReefer · Dec 31, 2016

Dosing Vibrant definitely kills off Chaeto.

jason2459 · Dec 31, 2016

My Turbos Aquatics ATS hasn't been impacted at all. Can see it was pushing out to the insides on both sides where it's flattened.

StrangeDejavu · Dec 31, 2016

NanaReefer said:
Dosing Vibrant definitely kills off Chaeto.

Curious if it's due to low nutrients or if the algae itself is being attacked.

NanaReefer · Dec 31, 2016

StrangeDejavu said:
Curious if it's due to low nutrients or if the algae itself is being attacked.

Not sure but I had a very health bunch and within 2wks of dosing 6ml 2x a week it started dying off. Within 4wks I had to remove it.
I'm now dosing 1x a week. Added a small bunch of chaeto and it's not doing very well. May have to remove it as well.

It's been my understanding that the Vibrant bacteria is short lived and does not multiply so I would think just 1x a week would do no harm to the chaeto.

blazedmasta · Jan 1, 2017

NanaReefer said:
Not sure but I had a very health bunch and within 2wks of dosing 6ml 2x a week it started dying off. Within 4wks I had to remove it.
I'm now dosing 1x a week. Added a small bunch of chaeto and it's not doing very well. May have to remove it as well.

It's been my understanding that the Vibrant bacteria is short lived and does not multiply so I would think just 1x a week would do no harm to the chaeto.

Yeah you would think so. I know I was able to grow cheato in my refugium with no issues. Started dosing 4ml 1x a week for GHA and notice my cheato started to get smaller and smaller. Just took all the cheato out and clean filter socks and pump of little peaces of cheato.

No3: 10ppm
No4: 0.03

rainmaker · Jan 1, 2017

Had a basketball size bunch oc cheato just took out the last little bit size of a tennis ball so hoping the vibrant will start working on my Dino's sense I have no other algae in tank now

drtrash · Jan 2, 2017

May do the same and pull it out for a few weeks, I am getting a surge of cyano and it's testing my patience

rainmaker · Jan 2, 2017

Might as well pull it out before it all dies if you have another place to it it

jason2459 · Jan 6, 2017

Several people have had a hard time finding bacteria in Vibrant. I've looked several times now, many more then I've posted, to keep double checking to make sure I'm seeing what I'm seeing is correct. The bacteria in there that I find most interesting are both the streptococcus and some type of very very small bacteria that's very hard to see. I certainly can not see it in my eyepieces. I have to close the iris a lot to almost closed which making using the eyepieces useless as it's way to dark. Then I use my camera software to increase the exposure a lot, bump up contrast and brightness to full. Even then it's tough to see and focus on. So, I really haven't been posting pics of them as you can't see them in still images against the other noise and in video its like I said can't focus on them but you can see their movement.

One thing I was looking for last night was some live streptococcus so here's a video of that but also went ahead and got some of the other bacteria in there that's about impossible to focus on. This is why I'd love to get a nice set of phase contrast lenses.

At ~1000x can see this streptococci moving around. Through the eyepieces I could easily make out the individual coccus that chain together to make the streptococci which I also confirmed with gram staining previously a few times. My eye pieces are stronger then the Camera's.

At 2000x it's tough to focus on the very small and started closing off the iris to help which starts to make the eyepieces useless. Through out you kind of see the other bacteria in there moving around but it's tough to see. It will either look like a tiny light or dark dot moving around.

jason2459 · Jan 6, 2017

To give a sense of scale.

1mm calibration slide. Each division is .01mm or 10um(micrometer/micron).

With the 100x lens or around 2000x in that second video here's the what it looks like to scale

CalibrationSlide100x by Jason, on Flickr

jason2459 · Jan 6, 2017

And if you're still having a hard time seeing it fast forward that second video to 3 minutes and 48 seconds and look out for this little critter I circled in this picture. Is well under 10um and probably around 1-5um.

VibrantBacteriaCircled by Jason, on Flickr