I have no air stone in there pumping air into the water if that's what you mean. I have a MJ1200 and MJ900 in there facing slightly upwards for good surface agitation. The rocks were dipped in a mix of water and vinegar. The container used was new and rinsed prior. Same with the container it is SUPPOSED to be cycling in now lol. So I see no way of anything being in there to kill it. Water temp is ~80 degrees Fahrenheit.