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Doing it in just a liter of tank water, the water clouded slightly (indicating massive increase in population)Just wondering about details again. Do you think the nitrate consumption is performed by pelagic bacteria or those associated with particulates in the aquarium?
Then the NO2 showed up.
My presumption is that there are many bacteria that can do this in the water or on surfaces. I also assume it's some portion of the population that scale up with the carbon dose that are doing the NO3->NO2.
My comment that it'd probably take less carbon if there were sand/rock is based on a guess that is easier to make space low in O2 at the substrate surface than in the bulk water.
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