I have a 3week old 75gal and I have zero ammonia, zero nitrite and zero nitrate. 55 lbs of live rock, 60 lbs of ocean direct sand, 4 fish, 1 cleaner shrimp, 1 urchin, hermit crab and conch snail. Had a 40 gal set up for 3 months and took all my bio filter, 40 lbs live rock and transferred to new tank. I use a Red Sea test kit and also had water tested at the LFS and the got zeros as well.