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Just as a side note you should know that once I’m happy with the initial results I can stop dosing organic carbon and silica and observe the difference, meaning that this tank will become a control,
Nope, this system won’t function as a control but will be another experimental starting point with an incompletely defined initial state with all the short comings of the first study. The plan is more like a meandering path through factor space with most of the factors unknown, uncontrolled and unmeasured.