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Lasse

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Closer look what´s happens when flow is constant but dosing rate and speed changes and how the ORP react on dosing ethanol in the plenum. Its clear that the system goes to a more reducing environment with each dose and that the amplitude of the oscillations becomes smaller as the dose decreases and/or the frequency increases while maintaining the 24-hour dose

2024-05-25 orp 2 bildjpg.jpg


Sincerely Lasse
 
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Adjusting things 250 km from the aquarium is normally not so good because it is impossible to check things on site. As you can see in lat post . I succeeded to dial it in rather good but.....

1716887346532.png


I lowered the dose Sunday at noon. The reason for the decreasing ORP readings could be low flow or dirty redox probe. I was home late Sunday evening and let it be for the day. Yesterday I decide to clean the pump and the probe and do some samplings below the DSB. Some result come up that I probably have to rethink some conclusions. The situation get worse - the plenum start to be more or less anaerobic - at least my probe report that.

1716887984314.png


After cleaning I decide to do some changes in the automation. Before I had use static parameters for the pump and dose of ethanol. I decided to keep the static for the dose but change the speed of the pump to dos 0.1 ml/minute. It means that it will take 2 minutes to dose 0.2 ml every 15 minutes (96 dose/24 hours) The software (and hardware) of GHL´s doser 2.x allow these settings with the latest FW. I had notice that with every dose - ORP decline. Therefore I programming the water pump to run when I dose and for 10 sec more. Using PL command delay off. However - I dose 2 minutes every 15 minutes - ORP can decline the other 13 minutes. If that happens - I have set the pump to react on decreasing ORP outside dose time. I set the redox control to nominal value 240 mV as a start, hysteresis 15 mV (blue). Controller on pulse variable and length of 5 min as max and fixed pause of 30 sec (black) This means that the controller is on if the real reading is below the nominal value and if it is far away from the nominal value it will run for max 5 minutes. Wait 30 seconds and if it still away from the nominal value it will run again. If it closer to the nominal value it will run shorter time and wait once again 30 sec. If it reach the nominal value during the cycle - it stops. This is a perfect controlling feature that allow you to have a much softer adjusting compared with the normal on/off controlling. The time I have set - you need to adapt to your system and how fast the system respond.

1716888905778.png


I have now to combine the two conditions for the pump to run - condition 1 - run when ethanol is dosed and condition 2 - run when the ORP is below the nominal value. It is done with a PL command as well.

PL 30 (pump runs when dosing ethanol)

1716889677505.png

PL 31 Combination with redox controller and the command OR

1716889798732.png


All of this has nor result in a rather smoth regulation of the flow and ORP

1716892038467.png

I also did an analyse of NH3/NH4, NO3m NO2 and PO4 both in the water below the DSB and in the DT. Remember - there was rather anaerobic compartment below the DSB a day before these analyses - hence probably the shocking NO2 concentration below the DSB. The diagram have also been adjuste - it was wrong figures for NO2 in the plenum before - forgotten a 0 on the wrong place

1716892515236.png


NH3/NH4 - still much of it (if not all) disappear when the water pass the DSB (reversed flow) Phosphate decrease. NO3 higher below the DSB than in the display tank for the first time and NO2 rather high in the plenum.

If the system works as it should and that I can control the ORP around 240 mV - I will take new measurements next week.

PO4 and NO3 in display tank during may 2024

1716893813243.png


Sincerely Lasse
 
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My measurements of the water below the DSB and water in the DT still produce figures that is difficult to answer. Last week has ORP below the DSB been around 250 mV - it means mainly an oxidative environment.


1717575241197.png

The coming week - I will run it around 200 mV

1717575315192.png

Yesterdays measurements - PO4 is on its rise again, NH3/NH4 goes down below the DSB. NO3 stable and NO2 goes down. If I look at the figures of NO3 and NO2 I think that the rise of NO3 below the DSB 27/5 is because NO2 interreference. Only more measurements can confirm or reject that theory.


1717575084053.png


Sincerely Lasse
 
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My fish list for the moment

4 Pyjama Cardinal - Sphaeramia nematoptera
1 Yellow-banded possum wrasse Wetmorella nigropinnata
1 Leopard wrasse Macropharyngodon bipartitus
4 Upsidedown Goby Probably Priolepis semidoliata
2 High Fin Perchlet Plectranthias inermis
1 Chelmon rostratus
1 Gobiodon Okinawae
2 Callogobius hasselti
1 Asterropteryx semipunctata
2 Assessor macneilli
1 Synchiropus ocellatus
2 Amphiprion ocellaris
7 Chrysiptera springeri
4 Chrysiptera parasema
1 sailfin blenny Emblemaria pandionis
2 molly miller blenny Scartella cristata
1 lemon or poison goby gobidon citrinus
1 small Bristletooth tang - rather alike Ctenochaetus truncatus but without the yellow eye - at least without it yet. the dots are there though.

Some of them new but most between 8 and 4 years in the aquarium.

38 fishes in a 80 gallon and I plan some more

Other animals

1 Stenopus hispidus
1 Rhynchocinetes durbanensis
1 Lysmata debelius and here
1 Percnon gibbesi
6 different urchins
30 + snails of different species
30 + hermits of different species
sea cucumbers and other critters

Sincerely Lasse
 
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Since a couple of months ago I have done some analyses of the "poor" water below my DSB. I pump spme water down below the DSB, water that slowly penetrate up through the DSB. I also pump some DOC (ethanol) into this plenum below the DSB. The DSB is around 800 mm (L) * 170 mm (W) * 200 mm (D) and consists of coral sand. I measure the ORP below the DSB. Positive ORP - more oxidative processes - negative ORP - more reducing processes. Higher positive ORP means that oxygen is present. The graph shows the average ORP, flow through the bed and added ethanol. The values isare a 7 days average prior that my sample was taken. The samples was analyzed for PO4, NO3, NO2 and total ammonia (NH3+NH4) with help of a Hanna Marine Master. The sample was taken below the DSN and filtrated through a 0.22 µ syringe filter. At the same time all these 4 compounds was also analysed from the display tank. Both flow and and amount of DOC was varied in 7 days periods

1719264950503.png


ORP seems to be connected to both flow and DOC addition. However 18/6 I changed from a wanted stable ORP and automatic increase or decrease of the Flow to a variable nominal value for ORP based on ORP in my Display tank. This have lead to a high variance of the ORP but a rather high average. Not sure what this mean yet - have to do some more analyzes the coming months

ORP below the DSB last week

1719261714717.png


I have shown some of my result in earlier posts but this time I will show them for each sample day.

1719261797853.png


1719261899616.png


1719261936797.png


1719261961979.png


1719261989979.png


1719262013672.png


and finally

1719262063779.png


I have today lower the ethanol dose with 1 ml a day. My goal is to have a concentration of NO3 in the DT around 5-6 mg/L. The high NO3 concentrations in the poor water 27/5 and 24/6 can be wrong and in reality only indicates nitrite interference with the analysis. This means that Hannas high NO3 can be sensitive to NO2 levels around + 0.3 mg/L

As usual - it is not possible to say so much about the importance of the result - however it rise some more questions. One is how it is in the middle of the sand bed. I have some ideas how to take some samples in the middle of the DSB.

One result is that I now know that where is part in the system where both total ammonia and nitrite occur.

Sincerely Lasse
 
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Did you supply that clam from your tank?
Yes - the only survivor of my four clams. I killed 2 - 1 is Sällström responsible for.

Sincerely Lasse
 
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