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It won't ruin the rock, but will kill any bacteria or other living creatures that are in or on the LR.
Thanks for the info! One rock is the size of a basketball. Can I take the rock out of the tank and put it in RO/DI fresh water? Will that ruin the rock?
I used Joe's Juice or any similar Aiptasia remover.
If you have alot of shrooms, cutting the rock won't be an option. Any little part of the foot that is missed will just become another shroom eventually.
If you can get the rock out full of mushrooms you could just take whole rock up to the LFS and trade for new rock + something something else.