I believe freezing would kill the bacteria, or substantially hinder them.Allan, I'm curious if you have tried freezing the concoction? This is the Finnish version, AB+ on ice made of bacterial mix. I would see this quite convenient way, for example freezing the concoction in disposable ice cube plastic bags and just throwing a cube or two to tank when needed.
The one time dose
Dead bacteria do not have the same effect as live bacteria. Perhaps you can try and let me know!
Sure, most likely I'll try the more long term storage route! It is more likely that the bacteria will be somewhat inactive in terms of multiplication after freezing, but killing all of them in regular household freezer is unlikely. Not planning to freeze them in liquid N2 or something silly like that
as long as you have room in your freezer it'll be fun to see!Sure, most likely I'll try the more long term storage route! It is more likely that the bacteria will be somewhat inactive in terms of multiplication after freezing, but killing all of them in regular household freezer is unlikely. Not planning to freeze them in liquid N2 or something silly like that
)
Yup, thicker, plumper tissue is another common effect. Just remember to feed more in conjunction to prevent bottoming out.More things I noticed on the concoction that I Forgot to mention:
Thicker skin on acropora. Originally this alarmed me as the skin was so puffy it made me think bacteria. But there was not discoloration so I believe this was positive.
Lower nutrients: this one makes sense as you’re probably dosing denitrifying bacteria in as well. Which is awesome because most coral foods raise nutrients and I really don’t like that. It’s lower my phos from .2 to .11 and my no3 from 38 to 24 so far. (Best thing since sliced bread
this is about 10 consecutive days on v2.
They also have a noticeable improvement in polyp extension the day after dosing Probidio Biodigest (not in concoction) almost the same as I noticed they reacted after the first day of dosing cipro.
As Allan mentioned, temperature matters. I did 2 different batches with one at 74F and one at 90F. The difference is huge. The batch at 90F had maybe 1000x the activity.
This will take further testing but the batch at 74F had a mix similar to my first ever batch with multiple bottled bacterias yet did not yield much more activity than you would find fresh out of a single bottle of bac.
The one at 90F had Aquaforest pro bio S and Prodibio Biodigest as the main bacterias added. The remaining ingredients consisted of a pinch of zeozym, calcium carbonate, coral sprint, min S, 1ml of aquavitro fuel, and a few drops of TM elimi NP. I am trying to make more a bacteria shot to fight dinos more than a feed but it worked regardless. Dino testing is still ongoing
I got a coffee mug warmer to keep the concoction to temperature. I used a coaster to try and keep it less than 100F and luckily it landed at 90F steady for 8 hours.
Here is what the results looked like under the scope:
74F
90F
They're going to die, I checked.
