I believe freezing would kill the bacteria, or substantially hinder them.
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I believe freezing would kill the bacteria, or substantially hinder them.
Is there any specific reason to keep the bacteria alive after making the concoction, if they in any case are going to be poured to the tank to be eaten by corals?I believe freezing would kill the bacteria, or substantially hinder them.
The one time doseAlso this might be a stupid question but do you do for the dose the 1 use dose on the bottle or times it by the amount of days you’re making it for. So far I’ve been doing the former.
Dead bacteria do not have the same effect as live bacteria. Perhaps you can try and let me know!Is there any specific reason to keep the bacteria alive after making the concoction, if they in any case are going to be poured to the tank to be eaten by corals?
Sure, most likely I'll try the more long term storage route! It is more likely that the bacteria will be somewhat inactive in terms of multiplication after freezing, but killing all of them in regular household freezer is unlikely. Not planning to freeze them in liquid N2 or something silly like thatThe one time dose
Dead bacteria do not have the same effect as live bacteria. Perhaps you can try and let me know!
as long as you have room in your freezer it'll be fun to see!Sure, most likely I'll try the more long term storage route! It is more likely that the bacteria will be somewhat inactive in terms of multiplication after freezing, but killing all of them in regular household freezer is unlikely. Not planning to freeze them in liquid N2 or something silly like that
Yup, thicker, plumper tissue is another common effect. Just remember to feed more in conjunction to prevent bottoming out.More things I noticed on the concoction that I Forgot to mention:
Thicker skin on acropora. Originally this alarmed me as the skin was so puffy it made me think bacteria. But there was not discoloration so I believe this was positive.
Lower nutrients: this one makes sense as you’re probably dosing denitrifying bacteria in as well. Which is awesome because most coral foods raise nutrients and I really don’t like that. It’s lower my phos from .2 to .11 and my no3 from 38 to 24 so far. (Best thing since sliced bread )
this is about 10 consecutive days on v2.
As Allan mentioned, temperature matters. I did 2 different batches with one at 74F and one at 90F. The difference is huge. The batch at 90F had maybe 1000x the activity.
This will take further testing but the batch at 74F had a mix similar to my first ever batch with multiple bottled bacterias yet did not yield much more activity than you would find fresh out of a single bottle of bac.
The one at 90F had Aquaforest pro bio S and Prodibio Biodigest as the main bacterias added. The remaining ingredients consisted of a pinch of zeozym, calcium carbonate, coral sprint, min S, 1ml of aquavitro fuel, and a few drops of TM elimi NP. I am trying to make more a bacteria shot to fight dinos more than a feed but it worked regardless. Dino testing is still ongoing
I got a coffee mug warmer to keep the concoction to temperature. I used a coaster to try and keep it less than 100F and luckily it landed at 90F steady for 8 hours.
Here is what the results looked like under the scope:
74F
90F
They're going to die, I checked.Whats the longest have you allowed the bacteria to culture? At 90 degrees i assume the bacteria eill be eating at a rapid rate and im womdering how long it would take for them to deplete the carbon source.
Whats the longest have you allowed the bacteria to culture? At 90 degrees i assume the bacteria eill be eating at a rapid rate and im womdering how long it would take for them to deplete the carbon source.
I currently run the AF probiotic method with ABEV and zeo stack for nutrient export. I started using this concoction yesterday. @Perry , I know you are quite familiar with AF, looking for suggestion on whether or not I should continue feeding the ABEV as directed or use that (which I did) only in the concoction and have the concoction replace everything with the AF Probiotic except the export of nutrients?
First reaction was pretty amazing with my torch and hammers... I have good polyp extension with my acros, but no growth... Checked parameters prior: PO4 .13; NO3 18. Little change (0.14) and NO3 same after a small dose. Did my routine water change yesterday. Did a second dose last night (larger) at lights out and PO4 was 0.14 and NO3 was 12.5 this AM (probably the water change at 20%). Excited to see how this works out.